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We asked you what local spots people should go to in your city instead of chain restaurants, and you delivered. We’ve complied suggestions for major metro areas so. An error occurred while setting your user cookie. Please set your. browser to accept cookies to continue. This cookie stores just a. ID; no other information is captured. Accepting the NEJM cookie is. A protocol for rapid generation of recombinant adenoviruses using the Ad. Easy system : Article : Nature Protocols. Top of page. Introduction. Replication- defective adenoviruses and biosafety issues. The complexity of adenoviral transcription units limits most recombinant manipulations to specific regions not essential for viral production, such as E1, E2. A, E3 and E4. First- generation adenoviral vectors replaced E1 genes with the desired transgene . Such E1- deleted vectors could be propagated in cell lines that express E1 gene products, such as HEK- 2. However, these vectors had relatively limited packaging capacity. Second- generation adenoviral vectors accommodated larger transgenes, reduced the cytotoxic effects in host cells and diminished the ability to elicit host immune response. For example, adenoviral vectors with deletions in E1, E3 and E4 (. Moreover, adenoviral vectors with deletions of E1 and E2 could prolong transgene expression and reduce cytotoxic effects. Although E3 is not essential for viral replication, specific packaging lines expressing the other deleted genes were required. In the extreme case, the whole adenoviral genome (except ITRs and the packaging signal sequences) was replaced by exogenous sequences, resulting in so- called gutted or gutless vectors, and the gene products required for viral replication and packaging were provided in trans. Only recently have genetic and. National Academy of Sciences. Graphical representation of the tetramer, dimer, and monomer of DsRed based on the x-ray crystal structure of DsRed. Gutless adenoviral vectors accommodated up to 3. DNA, exhibited significantly reduced host immune responses and achieved long- term expression of multiple transgenes in a single vector. Overall, there are several examples of the construction and use of conditionally replicating adenoviruses that promise delivery to specific cell types in vivo. In this protocol, we primarily focus on viruses with a deletion of both E1 and E3 (. These requirements include the use of biosafety cabinet hoods, the establishment of proper procedures for decontamination and disposal of liquid and solid wastes and the disinfection of contaminated surfaces and equipment. In addition, users should conduct a regular analysis of adenovirus stocks to exclude the presence of replication- competent adenoviruses in their preparations. Methods for generating recombinant adenoviruses. Three general approaches have been employed to generate recombinant adenoviruses. The first approach involves direct ligation of DNA fragments of the adenoviral genome to restriction endonuclease fragments containing transgenes. The difficulty of purifying large viral genomic DNA fragments, the low efficiency of large fragment ligations and the relative paucity of unique restriction sites in the adenoviral genome have made this approach unpopular. The second method uses site- specific recombinases. Accre Chemicals 2640 Midland Avenue Toronto, Ontario M1S1R7 Canada Accre Chemicals is a contract research organization of new drug discovery, pharmaceuticals. DESIGN PCR PRIMERS. BACKGROUND INFORMATION: For sites describing PCR theory, as well as companies marketing PCR products you might want to begin by visiting Highveld. The syndrome of inappropriate antidiuretic hormone secretion (SIADH) is a common cause of hyponatremia. We describe two infants whose clinical and laboratory. Nature Protocols 2, - 1236 - 1247 (2007) Published online: . A protocol for rapid generation of recombinant adenoviruses using.One useful example of such a method employs the Cre recombinase/lox. P site- specific recombination system. The third and most widely used method involves homologous recombination in mammalian cells or in microorganisms. This method requires a two- vector system, namely 'shuttle' and 'backbone' plasmids. A typical shuttle vector usually contains the 5. The resultant shuttle vector is subsequently recombined into the 'backbone' vector. This backbone provides most of the adenovirus genome but lacks genes essential for virus propagation in naturally occurring cells. After recombination, a single DNA molecule encoding all the genes necessary for virus production in packaging cells (but not in naturally occurring cells) is produced. Traditionally, the recombination process was achieved through homologous recombination in mammalian cells. However, this proved to be the rate- limiting step in the production of recombinant adenoviruses owing to the inefficient and somewhat unpredictable nature of homologous recombination in mammalian cells. Yeast and bacterial systems have, therefore, been explored for this purpose. Once a DNA molecule incorporating both the backbone and shuttle vector sequences has been generated in such microbial systems, the cloned DNA can be transfected into mammalian packaging lines for virus production. These approaches have made it possible to generate large quantities of recombinant adenoviruses in a timely and predictable fashion. The Ad. Easy system described in this protocol exploits the high efficiency of homologous recombination in a specific bacterial strain coupled with selectable antibiotic resistance markers to simplify recombinant vector production. The Ad. Easy system for regenerating recombinant adenoviruses. As outlined in Figure 1 and in TIMING, the Ad. Easy technology consists of three steps. BJ5. 18. 3 bacterial cells are deficient in certain enzymes that mediate recombination in bacteria (end. A, sbc. B- , rec. BC- , str. R)2. 1 but still permit efficient generation of stable homologous recombinants. The resultant plasmid is linearized by digesting with restriction endonuclease Pme. I and subsequently transformed into competent Ad. Easier cells, which are BJ5. Ad. Easy- 1. Recombinants are selected for kanamycin resistance and recombination is confirmed by restriction endonuclease analyses. Overall, the confirmed recombinant adenovirus plasmids are digested with Pac. I to liberate both inverted terminal repeats (ITRs) and transfected into HEK- 2. HEK- 2. 93 cells express recombinant adenovirus E1, allowing them to produce adenoviruses from backbone vectors without the E1 gene. Recombinant adenoviruses are typically generated within 1. The 'left arm' and 'right arm' represent the regions mediating homologous recombination between the shuttle vector and the adenoviral backbone vector. Alternative homologous recombination between two Ori sites is shown with dotted lines. PA: polyadenylation site; LITR: left- hand ITR and packaging signal; RITR: right- hand ITR. Adapted from ref. Full size image. Vectors for use in the Ad. Easy system. The Ad. Easy system can be used with any of four shuttle vectors. As shown in Figure 2a, p. Shuttle is the basic vector with the greatest capacity for accommodating foreign genes and/or the flexibility to customize a promoter for transgene expression. The p. Shuttle- CMV vector contains a cytomegalovirus (CMV) promoter to drive expression of the GOI. Both p. Ad. Track and p. Ad. Track- CMV incorporate an independent cassette expressing a GFP marker for convenient assessment of adenovirus transducing efficiency. However, these two vectors have a reduced capacity for accommodating foreign genes.(a) Four shuttle vectors with versatile selection of promoters and packaging capacities. Approximate locations of . Adapted from ref. Full size image. Two adenoviral backbone vectors can be used for adenovirus production (Fig. The commonly used p. Ad. Easy- 1 is an E1 and E3 double- deletion vector. Hence, Ad. Easy- 1- derived recombinant adenoviruses can be propagated in E1- expressing packaging cells, such as HEK- 2. E3 is not necessary for viral production). The p. Ad. Easy- 2 backbone vector is an E1, E3 and E4 triple- deletion vector. Thus, propagation of Ad. Easy- 2- derived vectors requires the use of packaging cell lines expressing both E1 and E4. As the E4 gene product is toxic to mammalian cells, its expression is usually controlled by inducible promoters. Several such E1/E4 packaging lines are available. However, because of the leakiness of most inducible systems, high E4- expressing cells are often lost after serial passages, and it is difficult to generate high- titer Ad. Easy- 2- derived viruses. Thus, in this protocol we focus on procedures for generating recombinant adenoviruses with the Ad. Easy- 1 system. For investigators wishing to express particularly long (or multiple) transgenes, we recommend gutless systems. Unlike Ad. Easy vector systems, which can tolerate replacements in the E1 and E4 regions only, gutless systems can tolerate much larger replacements in the adenoviral backbone. Although the Ad. Easy system has been made freely available to thousands of academic researchers, the system can also be obtained through commercial sources listed on the Ad. Easy website (http: //www. Top of page. Anticipated results. The Ad. Easy system has proved to be an efficient and robust technology for generating recombinant adenoviruses in thousands of laboratories worldwide. The key step is the generation of recombinants in Ad. Easier cells. Once these recombinants are obtained, generation of adenoviruses in HEK- 2. Depending on the transfection efficiency of HEK- 2. It usually requires two to three rounds of further amplification in HEK- 2. Cloning genes of interest into shuttle vectors entails standard molecular biology techniques. The user's experience in molecular cloning will determine the time required for this step. After subcloning GOI into a shuttle vector, the rate- limiting step is generally the production of the desired recombinants in Ad. Easier cells. With experience and high- quality competent Ad. Easier cells, it takes as few as 2 d to obtain and verify the recombinants. If every step works well, one can expect to obtain the initial virus lysates 2–3 weeks thereafter and the subsequent large- scale virus preparation in an additional 1–2 weeks (see TIMING). Further information can be found at the Internet resources listed in Box 1. 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Earlier this month in Beijing, a Chinese movie company announced an upcoming feature film called Dragon Force: So Long Ultraman. The CG movie stars robots, a dragon. Run Malware. Bytes to remove persistent malware. Process name: Windows Management Instrumentation. Product: Windows. Company: Microsoft. File: wmiprvse. exe. Security Rating: Windows. Failure of a provider caused the entire WMI service to fail; the next request to WMI restarted the service. To avoid stopping all the services when a provider fails, providers are loaded into a separate host process named Wmiprvse. Multiple instances of Wmiprvse. Local. System, Network. Service or Local. Service. The WMI core Win. Mgmt. exe is loaded into the shared Local Service host named Svchost. In other cases, wmiprvse. Read also the 4. 73 reviews. Polls computer for hardware/software inventory. Daniel spikes processor to %1. This file is attacked by Trojan. Gletta. A, it would eat all the memory on my win. Gary If found in windows\system. Trojans, if its in windows\system. Microsoft file (check the properties) and this should be left where it is. Russ WMI is very cool. Steph I found it in the system. Apparently a search on wbem with google reveals that it is associated with Sun Microsystem's Java. Nothing to fear. Pepsibot Keeps 'ecountering problems' and closing. Buggers some games. Used to run background tasks Wizard of Zo This appears to be launching a brand new files called tipyno. Fu Kerr It really is a windows process, but be wary, as it's possible to be used inside a network, to monitor your processes by a sysadmin. Tano This exe also appears to load when waupdt is running. It pauses when task manager is activated. It vanishes and reappears. Kaled XP SP2 is taking forever to start up and this seems to be one of the problems starts when runnning tasklist /svc in cmd prompt. Reign located in .\system. This program allows you to query/update information from the Common Information Model. It's basically a standardized database with standard namespaces which can be found across multiple OS's. It allows programmers/System Administrators to inventory/debug/etc. SMS 2. K3 has inventory tools which use WMI to monitor your computer. It's not *that* dangerous. It has built- in security to make you authenticate to windows but.. I included a link to Microsoft's website that gives much more information on WMI See also: Link- =(Ther. Mite)=- It's lunched from a Microsoft game used by Direct Play 9 See also: Link. Jeep same over here - dozens of wmiprvse. Server, generate 1. Server load and DHCP and WWW- Services refuse to start. Stupid Trojan acting like Blaster/Sasser (Crashes RPC). Even In safe mode & networking! Nate This file (or something else related to a folder on my system called srchasst) is causing ads to pop up when im doing searches on google. Like, something about freedictonary. Supposedly is there to facilitate Client/Server development schemes. Yeah On my PC, it's the Win XP one - but its run for no good reason, getting caught by my firewall: wmiprvse. I don't trust it, and have no problems terminating it. More info on WHY or for what its needed would be appreciated. Alley. Kat It is a legitimate file for sp. It keeps lagging my connection! It causes a two to five second connection lag. It's not much, but it makes me keep loosing connection in certain MMORPGs. Very annoying, and is more persistent than a virus. Only attempt of process to access web was when performing Windows Automatic Update for first time tonight. Seems harmless, 0% cpu and 4. Meg memory usage. Mick It, together with svchost. O all the sudden my cpu was running full tilt and i had a new process.. Adark. A h. 8s MS Not much, but it doesnt seem to do anything Pyrothekilla It is from Win. XP Service Pack. 2 Don Starts when you opent 3dmark and ends when you close 3dmark GFX I found it running right after booting up, then it vanished. Tim You see it with Winxp SP2 because Windows Firewall, and the Security Center uses it. Oprime ok, I know for sure it isn't part of xp. I don't have xp. 2 on ths machine and I have WMIPRVSE. EXE running in the background. Just goes to show that you can't really trust anything on these boards. Jesseq causes me to blue scrren/advanced system info had ? I noticed this 6 hrs after alarm clock was due to go off (I use WMP and Task Scheduler to run MP3s as an alarm clock). Zazu It is a standard windows system proces for updating the system J! Loaded with NEROVISION 3. Blacknote you can't simply terminate it with the taskmanneger it starts automaticly again poll Seems to have become activated after setting up SMS 2. DCOM- Server See also: Link. Axel. C continually locks the task bar and quick launch buttons and wont allow me access to the c drive Doubly. Hate. MS it wasnt in the directory you said it should be, i denied it internet access, well see what happens now Magic. Marker Slowed my dual core 1. Mhz system down to PIII 5. Mhz speeds - pausing/stopping this service did the trick christopher Appeared after installing Adobe Acrobat Reader 7. Eats up ALL my cpu time. Raffi When I stop it, the SP security center also stops. I keep it running Marco I am a blind computer user and I use the screen reading software, this wmiprvse. I have to many internet operations running Sita If you go to Windows Update , and then close de window . It brings up the cpu usage to 1. Very much annoying DH It's making my computer shut down Causes random popups with Google; destroyed display settings along with direct. Zuwxiv It seems to be dangerous. When I delete or rename the process, it comes again. I think it coul be a virus, but I didn. Lazaro Freire After updating Windows XP with SP2 my PC takes about 3 minutes to sort itself out before I can do anything. I had a look in the process information and it this CSRSS. Most annoying. There should be one with SYSTEM, the other effects your system and CPU to go up 1. Nate Windows Management Instrumentation. On XP SP2, the Windows Firewall + Connection Sharing Service depends on this one. Very annoying. Disable both in Adminstrative Tools/Services but first install a couple of real firewalls! By using industry standards, managers can use WMI to query and set information on desktop systems, applications, networks, and other enterprise components. Developers can use WMI to create event monitoring applications that alert users when important incidents occur. DNA9. 00. 0 I spot it after the firewall poped up, it was in the windows xp sp. IRNBRU i know that u can`t delete it or shutting down . Du. Ke. 4Tr. ANc. E Trend Micro asked me to delete it to help resolve a problem with Pc. Cillin and internet behind a router.. Their in system. 32/wbem and SP2 i. Tom legit part of winxp- sp. T- Rust it's a windows system process Wayne . Ignore this info, you don't want to be doing that trust me. If there is a masquerading virus with this file. Consultant Not really know what it's for but it starts up with XP and then it dissapears but when it's running it shurely hogs up alot of resources I wonder if this is the cause of my online gaming problems and causes my connection to freeze if i were you guys I would google it extensively and make sure if it's ok to delete otherwise you would be up a creek without a paddle if you do and your system could go apeshit The WMIPRVSE. EXE application crash when you add or remove a HDD on a Win. XP SP1 system, but no effects on the system. Rael Only causes problems when the is more than one copy in system. For Winsdows XP, It should be located in: C\windows\system. Delete all other copies and it shopuld fix the hangups. Multipble copies are usually caused by downloading of music, pictures and ebooks. Note: wmiprvsw. exe is the Sasser worm! The wmiprvse. exe file is located in the c: \windows\System. Novawatcher i dont have sp. MS products voodoolady where is the original wmiprvse. Note: The wmiprvse. System. 32 folder. In other cases, wmiprvse. W3. 2/Sonebot- B drops a copy of itself to the Windows System. WMIPRVSE. EXE i have it running in my taskmananger but its not in system. I think mine is the normal windows and not a virus found the virus i was looking for hotkeysvc. Jason i dont have sp. C: \Windows\system. Someone start with 3dmark 2. SE zoidberg This file uses more cpu when i use robocopy to sync 2 servers. Floris (www. Data. Smit. nl) It keeps shutting down my Norton Anti Virus and closing my computer. Tried everything.. Sterrenplukker Black Ice firewall indicates it was called from atprint. Webex Player. Vanishes after about a minute. MIke well, definetley not from SP2 for information, Xp home Edition, just reformatted and installed and its still here!! Well my management software warned me that this file wanted to start and since i have not installed anything that would bring this file along I count it as a virus and didn't allow it to start and I have'nt experienced any problems. RKelly When I tried to shut it down it keeped reappearing until I deleted it from system. Let's hope it won't appear again. Only ran for about a minute then it quit. Craig dunno about it but wmiprvsw is a virus (sasser i think). If you have trouble,check if it's not this one instead.. Then, it attacked my girlfriend and gave her Trojans. Then, it stole my car. At least, it is not that much of a problem like some viruses. I don't like my girlfriend that much anyway. The car, well, it is old. But the Pizza, now, that was a problem! It's like playing online game with 6. Pisses me off Avoid wmiprvse. If you find it anywhere else then you should be suspicious for sure. Its run with my windows and always demands to connect to the internet Amy Supports Windows Internet Connection Sharing and other such services. Without it running, ICS wont be able to operate correctly. It seems harmless enough. Cade we know nothing about this file but we think rob is the douchebag poofingers it is a real big one, chuck norris used it in one of his episodes bridge in system. Mld. 1. 00% safe on uninfected systems. Children of God - Wikipedia bahasa Indonesia, ensiklopedia bebas. Children of God (COG) yang belakangan dikenal sebagai Family of Loves (Keluarga Kasih), Keluarga, dan kini Keluarga Internasional adalah sebuah gerakan agama baru yang dimulai pada 1. Huntington Beach, California, Amerika Serikat. Ini adalah bagian dari Jesus Movement (Gerakan Yesus) pada akhir tahun 1. Sebagian besar anggotanya berasal dari gerakan hippie. Gerakan ini adalah salah satu dari banyak gerakan lainnya yang membangkitkan kontroversi sekte pada tahun 1. Amerika Serikat dan Eropa dan memicu kelompok anti- sekte terorganisasi pertama (FREECOG). Sementara gerakan ini bertumbuh dan meluas di seluruh dunia, demikian pula pesannya—keselamatan, Milenarianisme, revolusi rohani melawan dunia luar yang mereka sebut sebagai . Pada 1. 97. 4, gerakan ini mulai bereksperimen dengan metode penginjilan yang disebut Menjala dengan Lirikan (Flirty Fishing)—dengan menggunakan seks untuk memperlihatkan kasih Allah dan memenangkan anggota baru dan mendapatkan dukungan. Praktik ini dihentikan pada 1. Pendiri dan pemimpin mereka yang diakui sebagai nabi, David Berg, berkomunikasi dengan para pengikutnya melalui Surat- surat Mo (Mo Letters)—yaitu surat- surat yang berisikan petunjuk dan nasihat tentang berbagai topik rohani dan praktis—hingga ia meninggal dunia pada akhir 1. Setelah kematiannya, jandanya, Karen Zerby menjadi pemimpin Keluarga. Kehidupan seksual yang bebas dari kelompok ini, penerbitan dan distribusi tulisan- tulisan, foto- foto dan video mereka yang menganjurkan dan mendokumentasikan kehidupan seksual antara orang dewasa dan anak- anak, serta seksualisasi anak- anak, menyebabkan munculnya berbagai laporan tentang hubungan seksual antara orang dewasa dan anak- anak. Sejumlah penyidikan hukum dan akademik pada tahun 1. Mereka yang terbukti melecehkan anak- anak setelah Desember 1. Rodriguez kemudian melakukan bunuh diri. Hal ini mengejutkan anggota- anggota . Sampai batas tertentu, mereka mengidentifikasikan diri, dan akan dipandang oleh kebanyakan orang, sebagai orang Kristen fundamentalis, meskipun sebagian dari keyakinan dan praktik mereka yang lebih radikal tentu akan dianggap non- tradisional, atau bahkan sesat, oleh kebanyakan orang Kristen fundamentalis. David Berg dianggap sebagai seorang nabi oleh mereka, dalam arti sebagai orang yang menyampaikan pesan- pesan dari Allah, bukan terutama sebagai orang yang meramalkan masa depan, meskipun seringkali ia berusaha melakukan hal itu, dan kebanyakan ternyata tidak akurat. Mereka percaya bahwa jabatannya telah diturunkan kepada istrinya, Karen Zerby, pada saat kematiannya. List Yayasan Baby Sitter, PRT, Perawat Orang Sakit/Lansia ini sengaja saya buat untuk tempat para Mommies yang menggunakan yayasan penyalur bisa saling sharing. Wongsoyudan, bukanlah fanbase. Mereka yang mengikuti karya saya sejak lama mungkin sering mendengar/ membaca betapa saya tidak menyukai punya fans. Ziddu is a unique place for content distribution in your personal and professional life. It offers free cloud storage for your valuable documents, photos, videos and. Antarmuka pengguna pada Android didasarkan pada manipulasi langsung, menggunakan masukan sentuh yang serupa dengan tindakan di dunia nyata, misalnya menggesek. Download Mudah RPP dan Silabus SMP Kelas VII, VIII, dan IX KTSP - Semenjak peninjauan kembali kurikulum 2013 oleh kabinet Joko Widodo Kurikulum 2013 menjadi di tunda. Mereka menganggap tulisan- tulisan yang diterbitkan resmi dari mereka bedua, namun bukan segala sesuatu yang telah mereka tulis, sebagai bagian dari Firman Allah. Sementara ada pemahaman dalam gerakan ini bahwa semua firman tidaklah mempunyai signifikansi yang sederajat, pada kenyataannya semuanya itu penting untuk dibaca dan diperhatikan. Mereka percaya bahwa Amanat Agung penginjilan dunia adalah tugas dari setiap orang Kristen, dan bahwa hidup mereka harus dipersembahkan demi pelayanan kepada Allah dan orang lain. Mereka mempunyai sejumlah tingkat keanggotaan dan para . Mereka juga sangat menganjurkan anggotanya untuk mempunyai anak. Meskipun keluarga berencana mulanya sangat tidak dianjurkan, pilihannya diserahkan kepada setiap individu dan praktiknya ternyata cukup lazim. Sebuah ajaran sentral dari teologi mereka adalah . Secara sederhana, ajaran ini mengatakan bahwa bila tindakan seseorang dimotivasikan oleh kasih yang tidak egois dan penuh pengorbanan dan tidak dimaksudkan untuk menyakiti orang lain, maka tindakan itu sesuai dengan Kitab Suci, dan dengan demikian sah di mata Allah. Mereka percaya bahwa ajaran ini mengatasi semua hukum Alkitab lainnya, kecuali hukum yang melarang homoseksualitas, yang mereka yakini sebagai suatu . Mereka percaya bahwa Allah menciptakan seksualitas manusia, bahwa seksualitas adalah kebutuhan emosional dan fisik, dan merupakan sesuatu yang alamiah, dan bahwa hubungan heteroseksual antara orang yang dianggap dewasa menurut hukum dan sama- sama mau, adalah suatu keajaiban yang murni dan alamiah dari ciptaan Allah, dan diizinkan menurut Kitab Suci. Para anggota dewasa boleh berhubungan seks dengan sesama anggota dewasa yang berlawanan jenis. Remaja di atas usia 1. Hubungan seks antara anak- anak dan orang dewasa dilarang keras. Para anggota . Hal ini disebut . Tak seorangpun boleh dipaksa melakukan hubungan seks di luar kehendak mereka, namun anggota- anggota yang tidak . Sebelum peristiwa itu datang, mereka percaya bahwa dunia akan diperintah selama 7. Anti Kristus, yang akan menciptakan suatu pemerintahan sedunia. Pada pertengahan masa pemerintahannya, ia akan sepenuhnya dikuasai oleh Setan dan hal itu akan mempercepat suatu masa yang penuh dengan kesusahan yang dikenal sebagai Zaman Kesusahan Besar. Ini adalah suatu masa yang penuh dengan penganiayaan yang hebat atas orang- orang Kristen serta masa di mana banyak terjadi bencana alam dan bencana- bencana lain yang tidak alamiah. Pada akhir masa ini, orang- orang Kristen yang setia akan diangkat ke surga dalam suatu peristiwa yang dikenal sebagai Pengangkatan yang akan segera diiikuti oleh suatu pertempuran antara Yesus dan Sang Anti Kristus yang biasa disebut sebagai . Dalam pertempuran ini Anti Kristus akan dikalahkan. Yesus Kristus akan memerintah Bumi selama 1. Milenium. Pernyataan kepercayaan resmi . Meskipun sebagian dari keyakinan ini tidak baru bagi . Sebagian dari keyakinan ini adalah: Nubuat: Dalam istilah . Kelompok ini memberikan tekanan besar bahwa setiap anggota menggunakan nubuat secara langsung dan teratur untuk membimbing hidup mereka sehari- hari. Meskipun nubuat, yang juga disebut sebagai . Para pakar mencatat bahwa, dibandingkan dengan gerakan keagamaan baru lainnya, . Para penolong ini diyakini menyampaikan pesan- pesan ilahi yang mereka terima dalam nubuat dan juga terlibat dalam pertempuran rohani melawan Setan dan roh- roh jahatnya. Kunci Kerajaan: . Karena itu, para anggota . Kunci- kunci ini juga diyakini memberikan kekuatan bagi berbagai pesawat angkasa luar, dan dapat secara ajaib berubah menjadi pedang untuk menolong mereka dalam peperangan melawan roh- roh jahat. Mengasihi Yesus: ini adalah istilah yang digunakan para anggota . Dalam pemahaman mereka tentang Kitab Suci, para pengikut Kristus adalah pengantin- pengantin- Nya, yang terpanggil untuk mengasihi dan melayani- Nya dengan semangat seorang istri. Mereka mengambil teologi pengantin selangkah lebih jauh daripada kebanyakan orang Kristen dengan menganjurkan para anggotanya untuk membayangkan bahwa Yesus sedang melakukan hubungan seksual dengan mereka pada saat mereka melakukan hubungan seksual atau bermasturbasi. Para anggota laki- laki diajarkan untuk membayangkan diri mereka sebagai perempuan . Doktrin- doktirn in menganggap Akhir Zaman memengaruhi semua pengambilan keputusan jangka panjang. Pendiri gerakan ini sebelumnya adalah seorang pendeta Christian and Missionary Alliance (C& MA), David Brandt Berg (1. Moses David, Mo, Bapak David, dan . Mereka melakukan proselitisme di jalan- jalan dan membagi- bagikan literatur. Anggota- anggota baru yang bergabung dengan gerakan ini menghafalkan kitab suci, mengambil kelas- kelas tentang pengetahuan Alkitab dan diharapkan meniru kehidupan orang- orang Kristen perdana, sementara di pihak lain menolak agama Kristen dari aliran arus utama. Pada Januari 1. 97. David Berg memperkenalkan melalui surat- suratnya bahwa ia adalah nabi Allah untuk zaman ini dan dengan demikian meningkatkan wibawa rohaninya di dalam kelompok ini. Meskipun demikian, Berg mengakui kelemahan- kelemahan dan kegagalannya. Penyebaran Surat- surat Berg di jalan- jalan (yang disebut . Laporan- laporan tentang kelakuan menyimpang yang serius, penyimpangan—penyimpangan finansial, dan penyalahgunaan posisi oleh sejumlah pemimpin yang mapan, termasuk tantangan sejumlah orang terhadap FFing (Flirty Fishing atau Menjala dengan Lirikan), memaksa Berg menata ulang gerakannya. Ia memecat lebih dari 3. COG saat itu. Pergeseran ini dikenal sebagai . Sepertiga dari seluruh anggota meninggalkan gerakan ini, dan mereka yang tetap tinggal menjadi bagian dari gerakan yang ditata ulang, yang kini dinamai . Kebanyakan dari keyakinan kelompok ini masih tetap sama. Metode- metode kesaksian biasa termasuk penyebaran traktat dan literatur injil lainnya dari pintu ke pintu, kelas- kelas yang mencakup musik . Hal ini dimulai sebagai sebuah eksperimen pada tahun 1. Berg dan akhirnya diperkenalkan kepada seluruh anggota. Pada 1. 97. 8, cara ini dipraktikkan jauh lebih luas oleh anggota- anggota kelompok. Di daerah- daerah tertentu para pelaku ML menggunakan agen- agen eskort untuk bertemu dengan orang lain. Seperti dalam metode- metode kesaksian lainnya, orang- orang yang dijangkau melalu ML mendukung gerakan ini dengan murah hati. Meskipun ML dipraktikkan dalam waktu yang relatif singkat, sebagian orang yang dijangkau lewat ML tetap menjadi teman, pendukung, dan terus memberikan dorongan hingga sekarang. Menurut . Anak- anak yang dilahirkan akibat ML disebut sebagai . Pada akhir 1. 98. Bapak David mengusulkan bahwa batas- batas dalam mengungkapkan kasih Allah kepada orang lain kadang- kadang dapat lebih daripada sekadar memperlihatkan kebaikan dan melakukan perbuatan baik. Ia mengusulkan bahwa bagi mereka yang sungguh- sungguh membutuhkan kasih dan afeksi fisik, bahkan seks dapat digunakan sebagai bukti tentang kasih Allah kepada mereka. 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